Journal: Oxidative Medicine and Cellular Longevity

Article Title: Effects of Saccharides from Arctium lappa L. Root on FeCl 3 -Induced Arterial Thrombosis via the ERK/NF- κ B Signaling Pathway

doi: 10.1155/2020/7691352

Figure Lengend Snippet: The effects of ALR-S on oxidative stress and ERK/NF- κ B signaling pathway in FeCl 3 -injured HAECs. HAECs were preincubated with different doses of ALR-S (0.1, 0.2, and 0.4 mg/ml) or serum-free medium for 4 h and then stimulated with 0.4 mM FeCl 3 for 2 h. (a) SOD activity in HAECs, n = 6. (b) GSH activity in HAECs, n = 6. (c) LDH levels in culture medium of HAECs, n = 6. (d) NO levels in culture medium of HAECs, n = 6. (e) Fluorescence image of ROS in HAECs stained with DCFH-DA, n = 3. (f) Flow cytometry analysis of ROS in HAECs stained with DCFH-DA, n = 3. (g) Protein expression level of p-ERK, NOX4, and cytoplasmic P65 in HAECs, n = 3. (h) Protein expression level of nuclear P65 in HAECs, n = 3. # p < 0.05 and ## p < 0.01 compared to the control group; ∗ p < 0.05 and ∗∗ p < 0.01 compared to the FeCl 3 group.

Article Snippet: The membranes were blocked with 5% milk for 60~90 min at room temperature and were incubated at 4°C overnight accordingly with the primary antibodies specific for TM (Bioss Antibodies, Beijing, China), TFPI (Elabscience, Wuhan, China), TF, FPA (Abcam, Cambridge, Britain), PECAM-1, p-ERK (Cell Signaling Technology, Boston, USA), ERK, NOX4, P65, lamin B, and GAPDH (Proteintech Group, Wuhan, China).

Techniques: Activity Assay, Fluorescence, Staining, Flow Cytometry, Expressing, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Effects of Saccharides from Arctium lappa L. Root on FeCl 3 -Induced Arterial Thrombosis via the ERK/NF- κ B Signaling Pathway

doi: 10.1155/2020/7691352

Figure Lengend Snippet: The effects of ALR-S and U0126 on platelet adhesion to FeCl 3 -injured HAECs. HAECs were preincubated with ALR-S (0.4 mg/ml), U0126, or serum-free medium for 4 h and then stimulated with 0.4 mM FeCl 3 for 2 h. (a) The effects of ALR-S and U0126 on platelet fluorescence intensity, n = 3. (b) Protein expression levels of TF, TFPI, TM, and PECAM-1 in HAECs, n = 3. (c) Protein expression levels of p-ERK and cytoplasmic P65 in HAECs, n = 3. (d) Protein expression levels of nuclear P65 in HAECs, n = 3. # p < 0.05 and ## p < 0.01 compared to the control group; ∗ p < 0.05 and ∗∗ p < 0.01 compared to the FeCl 3 group.

Article Snippet: The membranes were blocked with 5% milk for 60~90 min at room temperature and were incubated at 4°C overnight accordingly with the primary antibodies specific for TM (Bioss Antibodies, Beijing, China), TFPI (Elabscience, Wuhan, China), TF, FPA (Abcam, Cambridge, Britain), PECAM-1, p-ERK (Cell Signaling Technology, Boston, USA), ERK, NOX4, P65, lamin B, and GAPDH (Proteintech Group, Wuhan, China).

Techniques: Fluorescence, Expressing, Control